Program Overview

With the siSPOTR tools you can:

  1. Identify candidate siRNA/shRNA sequences for your gene of interest with a low potential for off-targeting.
  2. Evaluate the off-targeting potential for any RNAi sequence.
  3. View a list of all predicted off-targets for siRNA candidates, along with information on the site-type composition and probability of being off-targeted.
  4. BLAST your sequence to search for siRNA-like on/off-targeting


This work is published in the journal of Nucleic Acids Research. It was developed by the Davidson Laboratory and the Institute for Clinical and Translational Science at the University of Iowa


For general questions or comments about the site, please email

Citation Information

Generation of hairpin-based RNAi vectors for biological and therapeutic application.
Boudreau RL, Davidson BL.
Methods Enzymol. 2012;507:275-96. Review.
PMID: 22365779 [PubMed - indexed for MEDLINE]
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siSPOTR: a tool for designing highly specific and potent siRNAs for human and mouse.
Boudreau RL, Spengler RM, Hylock RH, Kusenda BJ, Davis HA, Eichmann DA, Davidson BL.
Nucleic Acids Res. 2012 Aug 31. [Epub ahead of print]
PMID: 22941647 [PubMed - as supplied by publisher]

Rational design of therapeutic siRNAs: minimizing off-targeting potential to improve the safety of RNAi therapy for Huntington's disease.
Boudreau RL, Spengler RM, Davidson BL.
Mol Ther. 2011 Dec;19(12):2169-77. doi: 10.1038/mt.2011.185. Epub 2011 Sep 27.